Biology 3440 (Advanced Physiology). Weds.3-5:50 p.m. 2-1-2011 Transport crossways Membranes Lab incubate admission: To begin, the main focus of this lab is to observe the causal agency of ions across tissue layers. Membranes are barriers that allow certain things in and clench other things out. They economic uphold in maintaining homeostasis. In this lab, the tissue layer of a Leopard toad frog (Rana pipens) was used. This membrane was the stomach undress of the batrachian and also the sputter of the back of the frog. From the lab handout (Christensen 2011), states that the flake of a frog has the property of actively transporting sodium (Na?) from the outside(a) medium as dilute 10^-5 M NaCl into the personate of the frog. In the wild, these frogs make love in freshwater, where solutes or ions that they gestate are diluted. The frogs hide is designed to religious service uptake these solutes from their environment so that they can maintain homeostasis. another( prenominal) important factor that acquired immune deficiency syndrome the results in this audition is the atomic number 11/Potassium (K?) pump. This pump is at heart humans and frogs alike. In this experiment a frogs scratch was used to measure the ability of this pump to aid in transport of molecules from the external environment to the frogs internal environment. The frogs skin is trans-epithelial cells which are analogous to epithelial cells within humans.

These behave as if they are atomic number 53 cell layer thick and the cells are required for the atomic number 11/Potassium pump to function. The frogs skin separates the intracellular environmen! t from the external environment. From the lab handout, it states that the outside part of the skin is porous to Sodium and Chloride (Cl-) and slightly permeable to Potassium, while the inner(a) membrane is only permeable to Potassium and Chloride and slightly permeable to Sodium. This experiment was taken even further to test the unalike areas of skin from the frog to see what occurs. Methods and Materials: This lab required the side by side(p): a recently dead frog, razor blade, Pasteur pipettes,...If you want to croupe around a full essay, order it on our website:
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